Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Cdk9

Cell type

Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)

Attributes by original data submitter

Sample

source_name
Murine erythroleukemia (MEL) cells
cell line
non-induced C88 MEL cells
chip antibody
CDK9 C20 antibody (Santa Cruz Biotechnology, C20, sc-484)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10 ng of ChIPped DNA was end-repaired, ligated to adapters, size selected on gel (200±25 bp range) and PCR amplified using Phusion polymerase. Cluster generation was performed using the Illumina Cluster Reagents preparation. ChIP-Seq library was prepared according to the Illumina protocol (www.illumina.com).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
73036692
Reads aligned (%)
93.6
Duplicates removed (%)
22.2
Number of peaks
6313 (qval < 1E-05)

mm9

Number of total reads
73036692
Reads aligned (%)
93.4
Duplicates removed (%)
22.2
Number of peaks
6142 (qval < 1E-05)

Base call quality data from DBCLS SRA